This method actually measures the presence of the basic amino acid residues, arginine, lysine and histidine, which contributes to …

Note: The data below should not be used as a replacement of a standard curve. Because 10µL of the standard sample is added to a well, there is 0.010mL × … The principle of this assay is that the binding of protein molecules to Coomassie dye under acidic conditions results in a color change from brown to blue.

The standard protocol can be performed in three different formats, 5 ml and a 1 ml cuvette assay, and a 250 µl microplate assay.

Prepare a standard curve by plotting the average Blank-corrected 562 nm measurement for each BSA standard vs. its concentration in µg/mL. The standard curve method is the most widely used for real-time Q-PCR quantification analysis. standard curve. PROCEDURE • Wear gloves, goggles, lab coat, and work very carefully • Take your samples out of -20oC freezer to thaw • Prepare the stock BSA solution and dilutions for the standard curve (Table 3.1, prepare freshly) • Prepare the …

It is necessary to generate a standard curve for both the target gene and the reference gene each time the assay is run.

I use BSA for standard curve and plot graph on excel as polynomial curve having 3 order points on trend line with equation and regression as under. Use the standard curve to determine the protein concentration of each unknown sample. Remove the 1x dye reagent from 4°C storage

For greatest accuracy in estimating total protein concentration in unknown samples, it is essential to include a standard curve each time the assay is performed.
2.1 Standard Protocol 1. Example of Assay Data Table Create a tablewith the absorbance results obtained from the assay. Two common proteins used for standard curves are bovine serum albumin (BSA) and an immunoglobin (IgG). standard curve prepared using the protein standards (Table 2 and Graph 1). Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.


The Pierce BCA assay can The linear range of these assays for BSA is 125–1,000 µg/ml, whereas with gamma-globulin the linear range is 125–1,500 µg/ml. BSA is such a protein, and the Pierce Albumin Standards are the most convenient source of ready-to-use BSA standard.

The kit contains the BSA standard set and the 1x … 7. These two proteins have different amino acid compositions, which lead to a different standard curve and a slight difference in the final determination of the unknown protein concentration. For the calibration curve, pipet 5 uL in duplicate for each standard in the BSA standard set. LogPhase 600 Microbiology Reader Measures Microbial Growth Curves in Multiple Microplates June 9, 2020 - BioTek unveils a unique class of microplate readers with the new LogPhase 600 Microbiology Reader.


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